Thaum ntau tus neeg koom tes me me thawj zaug tau ntsib nrog cov hlwb, lawv tsis paub dab tsi txog qhov kev sim reagent consumables uas yuav tsum tau rau cell culture, thiab lawv paub tsawg heev los ntawm cov ntaub ntawv.
cell kab lis kev cai
Cell Culture Basic Reagents
(1) Nruab Nrab: Feem ntau, nws tau npaj los ntawm nws tus kheej nrog qhov nruab nrab tsis tiav ntxiv rau 10 feem pua FBS (fetal bovine serum), thiab qee zaum muab ob npaug rau cov tshuaj tiv thaiv kab mob ntxiv los tiv thaiv kab mob ntawm tes. Cov hom nruab nrab tshwj xeeb raug xaiv raws li hom xov tooj ntawm tes uas koj tab tom tsa.
(2) Trypsin: hu ua trypsin, siv los zom cov hlwb. Feem ntau, lub sijhawm zom zaub mov yog 1-2 feeb. Yog tias lub sijhawm luv dhau, cov hlwb yuav tsis zom tag nrho. Yog tias lub sijhawm ntev dhau lawm, cov hlwb yuav dhau los thiab puas.
(3) Feem ntau siv reagents: cawv, PBS tsis.
Cell Culture Basic Consumables
(1) Lub thawv kab lis kev cai ntawm tes: kab lis kev cai lub raj mis 25, 50, 250ml, thiab lwm yam Petri tais: 10cm, 6cm, thiab lwm yam. Qee tus yuav siv lub raj mis kab lis kev cai lossis petri tais diav, thiab lub peev xwm sib xws yuav raug xaiv raws li qhov xwm txheej.
Cell Culture Consumables
(2) Consumables: straws, pipettes, ntau yam pipettes thiab tswv yim, centrifuge raj, parafilm, thiab lwm yam.
Cell Freezing Reagents thiab Consumables
(1) Cov tshuaj Cryopreservation: feem ntau yog tsim los ntawm 10 feem pua DMSO ntxiv rau FBS ntxiv rau nruab nrab, qhov piv ntawm FBS thiab nruab nrab nyob ntawm hom cell, cov hlwb muaj nuj nqis feem ntau siv 90 feem pua FBS, thiab cov hlwb zoo tib yam siv tau 50 feem pua FBS ntxiv rau 40 feem pua. kab lis kev cai nruab nrab los npaj.
(2) General reagents rau kab lis kev cai ntawm tes xws li trypsin.
(3) Cov kav dej khov, cov raj centrifuge, centrifuges, straws, pipette tswv yim, microscopes, khov thawv, tub yees, kua nitrogen tank, thiab lwm yam.