Ntxiv nrog rau kev xaiv cov reagents tsim nyog, kev ua haujlwm nruj thiab kev siv cov cuab yeej precision, kev xaiv kom raug thiab siv cov khoom siv xws li microplates kuj yog tus yuam sij rau kev ua tiav ntawm kev sim rau kev nthuav tawm cov txiaj ntsig kev sim zoo meej!
Cov lus nug nquag nug txog microplates
Q1: Yuav ua li cas xaiv cov xim ntawm lub microplate?
1. Kev kuaj pom fluorescence
Cov phaj dub microwell tuaj yeem nqus lub teeb, yog li nws tuaj yeem cuam tshuam qhov cuam tshuam ntawm autofluorescence, thiab yog qhov tsim nyog rau kev tshawb nrhiav fluorescence nrog lub neej luv luv (nanoseconds rau microseconds) xws li GFP, fluorescein, Cy3 thiab Cy5. Rau kev tshawb pom ntawm lanthanide chelates nrog ntev ib nrab-lub neej (microseconds mus rau milliseconds), lub sij hawm daws teeb meem fluorescence nrhiav tau yuav siv tau kom tsis txhob muaj kev cuam tshuam ntawm autofluorescence. Dawb microplates feem ntau pom zoo, tab sis yog tias muaj kev cuam tshuam loj, koj yuav tsum siv cov microplates dub.
2. Luminescence nrhiav pom
Feem ntau cov dawb microplates nrog lub teeb pom kev zoo siab yog siv. Txhawm rau kom tsis txhob cuam tshuam, siv cov xim dawb (AlphaPlate), grey lossis dub microplates. Tsis tas li ntawd, phosphorescence (hais txog qhov tshwm sim uas ib yam khoom xws li lub phaj microwell khaws lub teeb tom qab irradiated nrog lub teeb xws li lub teeb fluorescent) kuj yog ib qho laj thawj rau kev txhim kho keeb kwm yav dhau. Dub microplates txwv cov teebmeem ntawm qhov tshwm sim no. Rau cov microplates dawb, ntxoov ntxoo lawv los ntawm lub teeb li 10 feeb ua ntej kuaj pom kom ua tiav qhov kev hloov kho tsaus nti kom txo tau cov nyhuv ntawm phosphorescence.
Q2: Cov lus nug txog kev cuam tshuam ntug
Cov ntaub ntawv hais txog hom thiab cov kev daws teeb meem ntawm ntug teebmeem hauv qab no:
①Temperature gradient: Nws tuaj yeem daws tau los ntawm kev kho qhov kub thiab txias ntawm microplate rau tib qhov ntsuas kub raws li lub ntsuas ntsuas ua ntej ntsuas.
② Evaporation: Phaj npog lossis zaj duab xis sealing tuaj yeem siv los tiv thaiv evaporation. Tsis tas li ntawd, kev teeb tsa cov av noo hauv lub incubator kom ze li 100 feem pua pab.
③ Raws li qhov pom ntawm cov nyhuv ntawm qhov kev sim ntawm tes, nws tau tshaj tawm tias ua ntej incubating lub microplate ntawm chav tsev kub rau 1 teev ua ntej muab lub microplate rau hauv 37 degree incubator tuaj yeem pab cov hlwb sib npaug.
Q3: 1/2 AreaPlate txhais li cas?
Piv nrog rau hom 96-zoo microplate, lub pore txoj kab uas hla yog li ib nrab ntawm cov microplate. Txawm tias cov kua ntim me me tuaj yeem ua kom ntseeg tau tias cov qauv mus txog qhov tob dua, tsis txhob muaj cov teeb liab qis los ntawm cov kua ntim tsis txaus.
Q4: Puas yog AlphaScreen/AlphaLISA (tshuaj ntsuam xyuas kev siv tshuaj) puas yuav tsum tau siv lub microplate tshwj xeeb?
AlphaPlate muaj nyob rau hauv dawb-dawb, xim nruab nrab ntawm cov dawb thiab grey microplates. Alpha thev naus laus zis yog siv los ua kom pom lub teeb pom kev pom teeb liab. Hauv kev kuaj pom luminescence, cov microplate dawb siv feem ntau, tab sis kev siv AlphaPlate tuaj yeem cuam tshuam kev cuam tshuam thiab txhim kho kev tshawb pom qhov tseeb.
pcr paj
Q5: Dab tsi yog tus yam ntxwv ntawm Yongyue Medical's 96-zoo PCR phaj?
1) Ua los ntawm cov khoom siv kho mob zoo-qib PP raw khoom, tsis muaj deformation, sealing kev ua tau zoo, raws li USP VI qib;
2) Cov khoom xim muaj xws li dawb thiab dub, pob tshab microplates, thiab microplates rau SBS cov qauv, uas yog tsim rau automation;
3) Cov khoom lag luam tau tsim tawm hauv 100, 000- theem purification Rhiav yam tsis muaj DNase, RNase, thiab endotoxin muaj kuab paug;
4) Ua los ntawm cov khoom siv kho mob zoo PP, tsis muaj deformation, sealing zoo, raws li USP VI qib;
5) PCR phaj tuaj yeem phim PCR phaj zaj duab xis thiab PCR phaj npog;
6) Cov khoom muaj cov khoom siv kho qhov muag zoo heev, huab cua nruj, thiab thermal denaturation stability;
7) Cov khoom tuaj yeem paub txog kev ua haujlwm siab, thiab sib xws nrog cov tuam txhab sib txawv thiab cov qauv ntawm cov phom thiab PCR cov cuab yeej.
8) Muaj plaub hom qauv ntawm daim tiab: ib nrab tiab, tsis muaj tiab, tiab siab thiab daim tiab tag nrho, uas tuaj yeem ua tau raws li kev xav tau sib txawv.