Tom qab kev kho saum npoo ntawm lub phiaj phaj, lub peev xwm ntawm cov protein khi tau zoo heev, uas tuaj yeem ncav cuag 300-400ng igG / cm², thiab qhov hnyav molecular ntawm lub ntsiab khi protein yog> 10kD. Kev siv hom ELISA phaj no tuaj yeem txhim kho rhiab heev, thiab tuaj yeem txo qhov concentration thiab ntau npaum li cas ntawm cov protein txheej. Qhov tsis zoo yog tias nws tsim cov tshuaj tiv thaiv tsis tshwj xeeb, antigen lossis cov tshuaj tiv thaiv tsis haum. Tom qab antigen los yog antigen txheej, non-ionic detergent tsis tuaj yeem kaw qhov chaw tsis muaj protein ntau, thiab cov protein yuav tsum tau siv los ua sealant.
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