Cov lus qhia ntawm chaw ua haujlwm yog cov lus qhia pipette. Cov khoom siv polypropylene raug xaiv, uas tuaj yeem ua kom tsis muaj menyuam los ntawm qhov kub thiab txias siab, cov phab ntsa sab hauv yog siliconized, thiab tsis kov cov kua los xyuas kom meej qhov tseeb ntawm cov qauv xa mus. Cia peb saib ntawm kev faib cov lus qhia ntawm chaw ua haujlwm:
Raws li cov khoom siv siv nrog pipettes, cov lus qhia feem ntau tuaj yeem muab faib ua: cov lus qhia txheem; lim tswv yim; cov lus qhia tsawg adsorption; tsis muaj cua sov cov lus qhia, thiab lwm yam raws li kev siv sib txawv.
1. Hom qauv: Nws yog cov lus qhia dav dav, uas tuaj yeem siv rau yuav luag txhua qhov kev lag luam pipetting, uas yog hom kev lag luam zoo tshaj plaws.
2. Hom lim: Nws yog ib qho khoom siv uas tsim los kom tsis txhob muaj kab mob sib kis, thiab feem ntau siv rau hauv kev sim xws li molecular biology, cytology, thiab virology.
3. Tsis tshua muaj adsorption hom: Rau kev sim nrog siab rhiab heev yuav tsum tau, los yog precious cov qauv los yog reagents uas yooj yim mus nyob twj ywm, cov tsawg adsorption hom yuav raug xaiv los txhim kho tus nqi rov qab. Qhov saum npoo ntawm qhov tsis tshua muaj adsorption tau raug kho hydrophobic, uas tuaj yeem txo qhov seem ntawm cov kua nplaum uas tsis tshua muaj zog hauv lub taub.
Lub voj voog txheej txheem ntawm qhov chaw ua haujlwm lub tswv yim suav nrog rau 6 kauj ruam: kev teeb tsa lub tswv yim - ntim ntim - ua ntej ntxuav lub taub hau - nqus dej - kua tso tawm - tshem tawm taub. Txhua kauj ruam muaj kev khiav hauj lwm specification uas yuav tsum tau ua raws. Cia peb saib nws kom meej.
1. Kev teeb tsa: Txoj kev teeb tsa raug raug hu ua rotary installation method. Txoj kev tshwj xeeb yog ntxig rau sab saum toj ntawm lub tes tsho dawb rau hauv lub taub (txawm tias nws yog lub taub hau loj lossis lub thawv ntim), thiab thaum nias maj mam, Tig lub pipette hauv qhov kov 180 degrees counterclockwise. Nco ntsoov tsis txhob siv lub zog ntau dhau, cia nyob ib leeg chop lub hau rau nruab, vim tias ua li ntawd yuav ua rau tsis muaj kev puas tsuaj rau lub pipette hauv koj txhais tes.
2. Kev teeb tsa muaj peev xwm: Qhov chaw muaj peev xwm raug muab faib ua ob kauj ruam, ib qho yog kev hloov kho ntxhib, uas yog, lub peev xwm muaj peev xwm hloov kho sai sai kom ze rau qhov koj xav tau los ntawm lub pob tawm; Qhov thib ob yog kev kho kom zoo, thaum tus nqi muaj peev xwm nyob ze rau koj tus kheej qhov kev xav tau Tom qab tus nqi tau teeb tsa, lub pipette yuav tsum tau muab tso rau hauv kab rov tav ntawm koj xub ntiag, thiab qhov ntim tus nqi yuav tsum maj mam hloov mus rau qhov xav tau los ntawm kev hloov lub log. , kom tsis txhob muaj kev cuam tshuam ntawm kev pom qhov yuam kev. Thaum teeb tsa lub peev xwm, muaj lwm lub ntsiab lus uas xav tau kev saib xyuas tshwj xeeb. Thaum peb kho los ntawm tus nqi loj mus rau tus nqi me me, nws yog txoj cai; tab sis thaum peb kho los ntawm tus nqi me me mus rau tus nqi loj, peb yuav tsum tau kho ntau tshaj li ib feem peb ntawm ib qho kev tig ua ntej rov qab los, vim tias muaj qee qhov sib txawv ntawm lub txee, uas yuav tsum tau ntim.
3. Ua ntej ntxuav lub taub hau: Tom qab peb nruab ib lub taub tshiab los yog ua kom tus nqi muaj peev xwm, cov kua uas yuav tsum tau hloov pauv yuav tsum tau nqus thiab tso tawm ob lossis peb zaug, kom cov yeeb yaj kiab ua kua homogeneous tuaj yeem tsim rau ntawm phab ntsa sab hauv. ntawm lub taub. , los xyuas kom meej qhov tseeb thiab qhov tseeb ntawm kev ua haujlwm pipetting, kom tag nrho cov txheej txheem pipetting muaj reproducibility. Thib ob, thaum nqus cov kuab tshuaj organic los yog cov kua dej tsis zoo, cov pa roj carbon monoxide yuav ua rau lub siab tsis zoo nyob rau hauv lub tes tsho dawb chamber, ua rau cov kua xau. Nyob rau lub sijhawm no, peb yuav tsum tau ntxuav ua ntej plaub mus rau rau lub sij hawm kom huab cua nyob rau hauv lub tes tsho dawb chamber. Thaum cov roj saturated, lub siab tsis zoo yuav ploj mus.
4. Kua aspiration: thawj nias lub pipette paug khawm mus rau lub No. 1 nres point, thiab ces muab tso rau hauv lub taub hau vertically rau hauv cov kua nto. Qhov tob ntawm immersion yog: P2, P10 tsawg dua lossis sib npaug li 1 hli, P20, P100, P200 tsawg dua lossis sib npaug li 2 hli, P1000 tsawg dua lossis sib npaug 3 hli, P5ML, P10ML tsawg dua lossis sib npaug 4 mm (yog tias lub immersion tob dhau lawm, lub hydraulic siab yuav muaj qee yam cuam tshuam rau qhov raug ntawm qhov nqus. Tau kawg, qhov tshwj xeeb immersion qhov tob yuav tsum tau raws li cov kua qhov loj ntawm lub thawv tuaj yeem tswj tau yooj yim), tso tawm. khawm smoothly, thiab nco ntsoov tsis txhob ceev heev.
5. Tshem tawm: Thaum tso tawm, lub taub hau nqus yog ze rau ntawm phab ntsa ntawm lub thawv. Ua ntej nias lub pob tawm mus rau No. 1 nres. Tom qab ib ntus luv, nias nws mus rau qhov nres thib ob. Qhov no tuaj yeem ua kom ntseeg tau tias tsis muaj kua seem nyob hauv lub taub hau nqus. . Yog tias cov kua seem tseem tshuav tom qab ua haujlwm no, koj yuav tsum xav txog kev hloov lub hau.
6. Tshem tawm lub hau: Cov hau kev tshem tawm yuav tsum tsis txhob muab sib xyaw nrog lub hau tshiab kom tsis txhob muaj kab mob sib kis.
