PCR phaj yog ib hom polymerase saw cov tshuaj tiv thaiv (Polymerase Chain Reaction, PCR), uas yog siv los ua cov cab kuj rau primers, Taq DNA polymerase, dNTP, template nucleic acid, Mg, tsis, thiab lwm yam koom nrog hauv cov tshuaj tiv thaiv amplification.
Cov khoom siv PCR phaj
Nws cov khoom siv feem ntau yog polypropylene (PP) niaj hnub no, uas tuaj yeem hloov kho kom zoo dua qub thiab qis kub hauv cov txheej txheem PCR, thiab tuaj yeem ua tiav qhov kub thiab txias kom tsis muaj menyuam. Txhawm rau kom ua tiav kev ua haujlwm siab dhau los ua ke nrog rab phom kab, tshuab PCR, thiab lwm yam., 96-zoo lossis 384-zoo PCR daim hlau yog siv ntau dua. Hom pawg thawj coj ua raws li SBS thoob ntiaj teb tus qauv, thiab txhawm rau hloov mus rau PCR cov tshuab ntawm cov tuam txhab sib txawv, raws li daim tiab tsim, nws tuaj yeem muab faib ua plaub hom qauv: tsis muaj tiab, ib nrab tiab, tsa tiab thiab daim tiab puv.
Cov xim ntawm PCR daim hlau
Muaj pob tshab thiab dawb, ntawm cov xim dawb yog tsim nyog rau hom tshiab ntawm lub sijhawm tiag tiag fluorescent quantitative PCR.
Kev siv PCR phaj
Nws yog dav siv nyob rau hauv lub teb ntawm noob caj noob ces, biochemistry, kev tiv thaiv kab mob, tshuaj, thiab lwm yam, tsis tsuas yog nyob rau hauv cov kev tshawb fawb theem pib xws li noob cais, cloning thiab nucleic acid ib theem zuj zus tsom xam, tab sis kuj nyob rau hauv kev kuaj mob ntawm cov kab mob los yog txhua qhov chaw uas muaj DNA. thiab RNA. Nws yog ib lub sij hawm thiab yooj yim-rau-siv chav kuaj. Saj.