Cov lus qhia thiab calibration ntawm pipettes
Laboratory pipette cov lus qhia thiab calibration
Cov pipettes uas muaj nyob rau hauv qhov chaw sim yog yooj yim rau kev khiav lag luam, tab sis kev ua tiav tsis yog ib txoj haujlwm yooj yim.
1. Yuav ua li cas xaiv ib tug haum ntau yam ntawm pipettes?
30 feem pua -100 feem pua ntawm qhov siab tshaj plaws ntim ntawm cov pipette yog qhov zoo tshaj plaws ntawm kev siv. Tsis txhob siv cov pipettes loj rau pipette me me ntawm cov qauv kom tsis txhob cuam tshuam qhov tseeb.
2. Yuav ua li cas xaiv lub peev xwm tsim nyog ntawm lub ntsis?
Xaiv ib thaj tsam ntawm 35 feem pua mus rau ~ 100 feem pua ntawm cov lus qhia lub peev xwm nominal rau high-precision pipetting. Hais kom muab cov txiaj ntsig zoo tshaj plaws thaum ncav cuag 50 feem pua ntawm lub peev xwm nominal.
3. Teem lub ntim ntim
Kev kho los ntawm ntau yam loj mus rau ib qho me me yog txoj kev hloov kho ib txwm, tsuas yog tig lub teev counterclockwise;
Thaum kho los ntawm ib qho me me mus rau ntau qhov loj, nws yuav tsum tau hloov kho kom siab tshaj qhov teev ntim ntsuas los ntawm 1/4 tig, thiab tom qab ntawd rov qab mus rau qhov ntim ntim, thiaj li tshem tawm qhov sib txawv ntawm cov tshuab thiab xyuas kom meej qhov tseeb ntawm cov pipette.
4. Sib sau ua ke lub pipette taub:
Ntxig lub pipette vertically rau hauv lub ntsis, tig nws ib nrab tig sab laug thiab sab xis, thiab ceev nws.
Lus Cim: Tsis txhob ntaus lub pipette nrog lub pipette. Lub sijhawm ua haujlwm ntev yuav ua rau qhov chaw ntawm lub pipette loosen vim qhov cuam tshuam. Hauv qhov xwm txheej hnyav, lub pob qhov rooj kho qhov ntsuas yuav daig.
5. Cov dej nqus thiab tso tawm
Lub taub ntawm lub taub yog raus rau hauv cov kua nto rau 1-6 hli, thiab qhov tob ntawm lub taub hau yog hais txog thaum pipettes nrog ntau qhov sib txawv yog cov kua aspirating:
Tsis tas li ntawd, pre-rinsing lub pipette taub hau ua ntej aspiration kuj tseem ceeb heev. Feem ntau, nws raug nquahu kom yaug 3 zaug kom ntseeg tau tias qhov tseeb thiab qhov tseeb ntawm cov raj xa dej, vim tias ib txheej ntawm cov qauv yuav nyob twj ywm rau ntawm phab ntsa sab hauv ntawm lub taub hau pipette, uas yuav ua rau muaj kev ua yuam kev hauv qhov ntim me me. Kev ywg dej yog qhov tseem ceeb tshwj xeeb tshaj yog thaum tso cov kua dej tsis haum.
Nco ntsoov plaub lub cim mantra "slow thiab tas li ceev" thaum nqus thiab tso kua dej, thiab maj mam nqus thiab tso tawm maj mam ntawm qhov nrawm tas li. Nyob twj ywm rau 1-3s tom qab lub tshuab nqus tsev thiab tso tawm ua haujlwm, txhawm rau tiv thaiv qhov kev tso tawm tam sim ntawd los ntawm kev nqus sai heev thiab maj nrawm rau hauv cov kua dispenser kom corrode lub plunger thiab ua rau huab cua to; Thaum nqus cov kua, ua haujlwm ntsug. Lub taub yog txhim khu kev qha nyob rau sab hauv phab ntsa ntawm lub thawv.
6. Tom qab pipetting tiav lawm
Tom qab kev ua haujlwm ntawm pipette tiav lawm thiab lub hau pipette tau muab tshem tawm, nws yuav tsum tau muab dai rau ntawm rab phom tshwj xeeb. Cov tub ntxhais kawm uas ua raws li kev ua tau zoo tuaj yeem kho qhov ntsuas kom siab tshaj plaws thiab cia lub caij nplooj ntoos hlav rov qab mus rau tus qauv kom ntev lub neej kev pab cuam ntawm lub pipette. Lub pipette nrog cov kua nqus dej yuav tsum tsis txhob muab tso rau hauv tiaj tus, vim tias cov kua hauv lub taub hau tuaj yeem yooj yim kis rau sab hauv ntawm lub cev phom.
7. Yuav ua li cas kom ntseeg tau qhov tseeb ntawm cov kua thaum nqus cov kua nplaum lossis cov qauv tsis haum nrog phau ntawv pipette?
Ib qho txuas txuas txuas ntxiv nrog lub plunger sab nraud yog pom zoo rau kos cov qauv viscous. Yog tias koj siv lub piston phau ntawv pipette, koj tuaj yeem siv txoj kev thim rov qab. Thaum aspirating kua, nias lub suction thiab tawm khawm mus rau qhov thib ob nres, thiab nias lub pob rau thawj nres thaum tawm, uas tuaj yeem ua kom paub tseeb qhov tseeb ntawm qhov nqus ntim.
8. Yuav ua li cas xyuas seb puas muaj cov xau
Tom qab aspirating, nres hauv cov kua rau 1-3 vib nas this los soj ntsuam seb cov kua theem hauv lub ntsis poob; yog tias cov kua dej poob, thawj zaug xyuas seb puas muaj teeb meem nrog lub hau. Paub meej tias lub taub hau nqus dej yog OK, tej zaum yuav muaj teeb meem nrog lub piston sib dhos, thiab koj yuav tsum hu rau cov chaw tsim khoom cov neeg ua haujlwm tu.
9. Thaum twg kuv yuav tsum tau rov qab kho dua?
1-2 calibrations ib xyoos yog pom zoo nyob ntawm qhov zaus ntawm kev siv.
Pipette pipetting voj voog ua haujlwm cov kauj ruam: 1. Pipette tip installation; 2. Kev teeb tsa ntim; 3. Pre-ntxuav pipette taub; 4. Kua aspiration; 5. Kua paug; 6. Tshem tawm lub taub hau
