Cell shaping muaj cov kauj ruam ntawm kev txhaj tshuaj ntawm tes, shaping, thiab tau txais. Cov kab mob hauv lub cell kab lis kev cai yuav tsum loj hlob thiab loj hlob mus rau ib qho twg. Lub sijhawm no, nws yog ib qho tsim nyog yuav tsum tau cia siab rau trypsin zom cov hlwb ntawm cov phab ntsa sab nrauv ntawm lub khob thiab mam li hnov nws. Nyob rau hauv cov ntaub ntawv ntawm enzymes, lub sij hawm digestion yuav tsum tau tswj.
T75 cell kab lis kev cai hwj
Trypsin belongs rau lwm chav kawm ntawm trypsin, uas tuaj yeem degrade cov proteins ntawm cov cell membranes fused rau cell culture flasks. Los ntawm dissolving cov protein nyob rau hauv qhov sib thooj, cov protein nyob rau hauv kev sib tshuam ntawm cov proteins yog yaj, thiab lub cell ua kheej kheej vim yog kev txhawb zog ntawm lub cell thav duab ntawm nws tus kheej sab hauv, yog li cais cov hlwb. Qib ntawm trypsin digestion yog qhov tseem ceeb hauv kev tsim ntawm tes: kev zom zom ntau dhau ua rau cov khib nyiab ntawm tes, cov khib nyiab dub nce, cov hlwb yuav poob tag nrho, ua rau muaj kev puas tsuaj loj ntawm tes, thiab qee lub hlwb ntab, nrog rau cov khoom pov tseg trypsin Outflow; yog tias kev zom zaub mov tsis txaus, nws yog qhov nyuaj rau cov hlwb kom tawg los ntawm cov phab ntsa sab hauv ntawm lub raj mis, thiab qhov nruam tshuab thiab ntaus yuav ua rau cov cell ua haujlwm puas.
cell culture khob
Serum protein kab lis kev cai kua raj mis
Hauv cov tsev sib txawv lossis cov hlwb, cov nyhuv ntawm pancreatic enzymes tsis zoo ib yam. Cov kev ua ntawm pancreatic enzyme dispersed hlwb kuj muaj feem xyuam rau nws cov concentration nqi, kub thiab cov nyhuv ntev. Thaum pH yog 8. Thaum thov trypsin, qhov concentration, kub thiab ntev yuav tsum tau tswj kom zoo los tiv thaiv kev puas hlwb los ntawm kev zom zaub mov ntau dhau. Vim tias Ca2, Mg2 thiab cov protein hauv cov ntshav, protein grams txo cov haujlwm ntawm pancreatin, yog li thaum npaj cov kua dej ntawm pancreatin, siv BSS yam tsis muaj Ca2, Mg2, xws li: D-Hanks tov. Thaum nres kev zom zaub mov, trypsin tuaj yeem tso tseg nrog cov ntshav qab zib cov kab mob hauv nruab nrab lossis trypsin retarder kom tsis txhob muaj cov txiaj ntsig ntawm trypsin ntawm cov hlwb.
kab lis kev cai khob
T25 cell kab lis kev cai hwj
Tsis tas li ntawd, yog tias nws yog ib lub xovtooj tshiab, koj tuaj yeem siv qhov qis dua ntawm trypsin los tshawb txog lub sijhawm zom zaub mov ua ntej, thiab saib seb lub hlwb puas hloov pauv txhua feeb, thiab ua cov ntaub ntawv zoo. Tag nrho cov nyob rau hauv tag nrho cov, thaum tau txais cov hlwb nyob rau hauv lub cell kab lis kev cai hwj, nws yog ib qhov tsim nyog los tswj lub sij hawm trypsinization, thiaj li ua tau zoo tswj lub viability ntawm lub hlwb.
