Niaj hnub no, cia peb tham txog lub ntsiab tseem ceeb hauv kev sim PCR - khoom siv. Raws li tus neeg tuaj saib PCR nquag, nws yuav tsum nkag siab txog cov yam ntxwv thiab cov lus hais txog PCR cov khoom siv.
Muaj ntau yam PCR consumables nyob rau hauv lub khw, nrog rau cov hom, specifications, xim, thiab lwm yam., uas yog dazzling. Cia peb nkag siab nws ib kauj ruam zuj zus.
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1. Khoom siv
Feem ntau, cov khoom siv raw ntawm PCR consumables yog polypropylene. Nws yog cov khoom siv hluav taws xob zoo-resistant inert. Qhov kub ua haujlwm yog -30 txog 140 degree, uas ua rau nws tuaj yeem tiv taus lub zog thermal hloov thaum lub sij hawm thermal voj voog ntawm PCR cov tshuaj tiv thaiv, thiab ua lub luag haujlwm zoo hauv kev sib khi thiab tiv thaiv cov tshuaj hauv PCR cov tshuaj tiv thaiv.
Kawm paub ntau ntxiv txog polypropylene
2. Xim
Feem ntau yog cov hlab dawb thiab pob tshab PCR raj. Hauv kev sim PCR ib txwm muaj, tsis muaj qhov sib txawv ntawm ob. Txawm li cas los xij, hauv tib lub sijhawm tiag tiag / qPCR qhov kev sim, cov hlab dawb tau zoo dua thiab zoo ib yam rau qPCR cov cuab yeej nrog cov teeb liab apical nyeem (saib daim duab A thiab B hauv qab). vim li cas thiaj zoo li no?
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A: qis Ct qhov tseem ceeb rau cov raj dawb piv rau cov raj ntshiab; B: zoo dua repeatability nrog cov hlab dawb.
Cov ntaub ntawv tseem ceeb ntawm qPCR yog tus nqi Ct, thiab cov teeb liab fluorescence yog cov ntaub ntawv siv los suav cov nqi Ct, yog li nws tseem ceeb heev seb cov fluorescence hauv lub raj tuaj yeem xa mus rau lub ntes tag nrho. Lub phab ntsa ntawm lub raj dawb tiv thaiv cov fluorescence tsis dhau los ntawm lub raj phab ntsa thiab refracting (Daim duab A hauv qab), yog li tiv thaiv cov fluorescence los ntawm kev xa mus rau cov cua sov thaiv (piv txwv li, phab ntsa ntawm lub qhov hlau qhov twg lub raj yog muab tso rau) thiab raug absorbed. los yog inconsistently reflected los ntawm lub block. Lub teeb liab muaj zog thiab muaj zog ntau dua fluorescent tau sau rau ntawm qhov kawg ntawm lub raj dawb piv rau lub raj ntshiab (Daim duab B hauv qab).
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A: Kev hloov pauv ntawm cov teeb liab fluorescence hauv lub raj pob tshab thiab lub raj dawb; B: Kev sib piv fluorescence siv tom qab ntxiv cov nyiaj fluorescein tib yam.
Yog li yog tias koj siv cov cuab yeej nyeem qPCR saum toj kawg nkaus, sim cov raj dawb.
3. Zoo (Lean lossis Tsis Lean)
Qhov tseem ceeb ntawm consumable quality yog hais txog ntawm no. qPCR consumables yuav tsum tsis muaj cov kab mob thiab inhibitors. Kev kub siab thiab hluav taws xob tshem tawm cov kab mob thiab DNases, tab sis tsis yog plua plav thiab DNA residue, uas tuaj yeem cuam tshuam PCR cov tshuaj tiv thaiv lossis tsim cov kev ua kom tsis muaj zog. Cov khoom muaj absorbance, thiab txawm tias cov pa phem me me yuav nqus cov fluorescence hauv cov tshuaj tiv thaiv kab mob thiab cuam tshuam rau kev sau cov cim fluorescence. Ib qho exaggerated version ntawm qhov kev sim tau siv los ua kom pom qhov cuam tshuam ntawm impurity contamination ntawm qhov kev sim. Raws li pom hauv daim duab hauv qab no, ib qho impurity tau muab tso rau hauv ib qho ntawm cov tshuaj tiv thaiv qhov dej ntawm octave. Raws li qhov xav tau, qhov kev nthuav dav nkhaus tau pom tias muaj qhov tsis sib xws.
Cov impurities hauv PCR cov hlab ntsha
↑ Impurities nyob rau hauv cov tshuaj tiv thaiv raj
8 kab
↑ Rov ua dua cov tshuaj tiv thaiv rau 8 lub raj, sab laug zoo impurity "deviates" qhov nkhaus.
Lus Ceeb Toom: Ceev faj tsis txhob tso cov khoom siv rau saum huab cua ntev ntev. Tom qab tshem tawm cov khoom siv, nco ntsoov kaw lub hnab ntim sai sai kom tsis txhob kov nws ntawm tes liab qab. Yog tias ua tau, ua haujlwm ntawm lub rooj zaum huv.
4. Muaj peev xwm / Loj / Flux
Lub ntim txiav txim siab npaum li cas tus qauv (cov tshuaj tiv thaiv ntim) tuaj yeem thauj khoom. Kev siv tshuaj ntau dhau yuav ua rau txo qis kev hloov pauv hluav taws xob, nchuav, lossis sib kis; underdosing yuav ua rau evaporative qauv poob. Cov cua sov thaiv ntawm qPCR ntsuas kuj tseem yuav txiav txim siab txog cov khoom siv thiab cov tshuaj tiv thaiv kab mob loj. Kev nkag siab yooj yim nkag siab, nws yog qhov siab tshaj plaws ntawm cov qauv uas tuaj yeem thauj khoom hauv ib qho kev sim. Common peev xwm thiab flux specifications muaj nyob rau hauv cov lus hauv qab no:
Ib lub raj / yim kab {{0}}.2mL, 0.1mL
{0}}zoo phaj 0.2mL, 0.1mL
{{0}} zoo phaj 0.2mL
Lub peev xwm specifications kuj feem ntau muab faib ua tus qauv thiab qis hom, raws li qhia hauv qab no. Tsawg profile consumables muaj qhov siab qis. Qhov kev tsim luv luv no txo qis qhov chaw saum toj no cov tshuaj tiv thaiv kab mob ("tuag ntim" - qhov tsis muaj txiaj ntsig), txo cov kev cuam tshuam ntawm evaporation thaum lub sij hawm thermal cycling, thiab ua kom lub cua sov module qhwv nyob ib ncig ntawm nws mus rau ntau dua los txhim kho cov cua sov ua haujlwm. Yog li ntawd, cov raj qis profile kuj hu ua "ceev" raj lossis daim hlau.
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Lus Ceeb Toom: Cov tshuaj tiv thaiv uas nquag siv yog 20 µL, thiab nws raug nquahu kom siv lub raj me me ntawm 0.1 mL lossis ntim me me.
5. Phab ntsa thickness
Lub thickness ntawm lub raj phab ntsa ncaj qha cuam tshuam rau thermal conductivity. PCR consumables yuav tsum muaj cov phab ntsa tuab tsis sib xws los muab cov cua sov sib xws. Lub phab ntsa ultra-thin raj yog 50 feem pua thiner dua li tus qauv version, uas txuas ntxiv txo cov thermal barrier thiab coj sai dua thiab zoo dua cov lus teb. Yog li ntawd, txhawm rau ua kom muaj txiaj ntsig zoo ntawm PCR cov tshuaj tiv thaiv thiab xyuas kom ruaj khov thiab muaj txiaj ntsig zoo, cov khoom siv PCR sib xws thiab ultra-nyias yog qhov kev xaiv zoo tshaj.
6. Pob tshab
Caps thiab sealing zaj duab xis rau qPCR xav tau qhov pom kev pom tseeb kom pom kev nkag mus thiab xa cov teeb liab fluorescent thaum txo qis kev cuam tshuam (rau cov ntawv nyeem qPCR saum toj kawg nkaus). Qhov siab dua qhov pom ntawm lub raj phab ntsa, nws yuav cuam tshuam rau qhov kev sim. Qhov laj thawj tau piav qhia saum toj no. Ib txwm PCR tuaj yeem tsis quav ntsej qhov no.
7. Cov tiab
Daim tiab ntawm PCR phaj hais txog qhov txuas ntxiv ntawm lub phaj. Thaum tsim cov tshuaj tiv thaiv kab mob, daim tiab ua haujlwm ruaj khov rau cov txheej txheem pipetting thiab muab cov cuab yeej siv zog thiab tuav thaum ua ke nrog cov chaw ua haujlwm automated. Muaj peb hom tiab, ib nrab tiab thiab daim tiab tag nrho (xws li A, B, thiab C hauv daim duab hauv qab).
Skirtless: Yuav siv tau nrog feem ntau PCR lossis qPCR cov cuab yeej, tab sis tsis yog rau cov ntawv thov siv. Yooj yim txav thaum lub raj xa dej, tsis ruaj khov heev, yuav tsum tau siv nrog lub phaj tuav.
Ib nrab Skirts: Zoo tagnrho rau kev siv cov khoom siv nrog zoo pipetting stability.
Daim Ntawv Qhia Tag Nrho: Zoo tagnrho rau kev siv tshuab. Zoo txhua yam muaj zog, haum rau PCR cov cuab yeej nrog protruding modules, siab stability thaum pipetting.
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Lub tswv yim: Qhov tseem ceeb ntawm daim tiab yog ntau dua los pab txhawb kev lag luam thiab kev ua haujlwm batch, ua ke nrog cov neeg hlau caj npab. Tej zaum nws yuav tsis xav tau hauv cov chaw tshawb fawb ib txwm siv, tsis muaj daim tiab txaus.
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8. Parafilm
PCR phaj sealing zaj duab xis yog ua los ntawm cov yas ntshiab lossis aluminium ntawv ci. Adhesive pob tshab zaj duab xis yog ntau dua, haum rau 96 lossis 384 daim hlau zoo, thiab yuav tsum muaj lub teeb pom kev zoo txaus. Thaum sealing zaj duab xis, ib daim npav me me yuav raug siv los nias zaj duab xis nyob ib ncig ntawm lub qhov sealing kom tsis txhob muaj cua nkag.
Adhesive aluminium films tej zaum yuav tsawg dua. Txawm hais tias tsis haum rau qPCR, nws yog qhov khoom siv zoo tshaj plaws rau cov qauv teeb pom kev zoo. Vim tias nws tuaj yeem raug xuas nrig ntaus, nws yooj yim hloov cov qauv hauv PCR (rau cov pa PCR).
PCR phaj sealing zaj duab xis
Cov ntsiab lus
Raws li tus cab kuj ntawm PCR system, consumables yog ib qho tseem ceeb heev los txhim kho qhov zoo ntawm kev sim cov ntaub ntawv. Kuv vam tias qhov kev sib qhia no tuaj yeem pab koj nkag siab qee cov lus qhia ntawm PCR consumables thiab muab kev pab rau kev xaiv cov khoom siv zoo dua.
