Raws li cov khoom siv siv hauv pipettes, cov lus qhia feem ntau muab faib ua cov lus qhia txheem, cov lus qhia lim, cov lus qhia tsis zoo adsorption thiab cov lus qhia tsis muaj cua sov raws li lawv siv. Cov lus qhia txheej txheem yog cov lus qhia siv dav tshaj plaws. Cov lus qhia dav siv tau rau yuav luag txhua qhov kev ua haujlwm pipetting, uas yog hom kev lag luam ntau dua. Cov lus qhia lim dej yog cov khoom siv tsim los tiv thaiv kev sib kis. Feem ntau siv hauv molecular biology, cytology, virology thiab lwm yam kev sim. Rau cov kev sim uas yuav tsum tau muaj siab rhiab heev, los yog cov qauv zoo nkauj los yog cov tshuaj reagents yog cov residue, cov lus qhia tsis tshua muaj adsorption tuaj yeem xaiv los txhim kho kev rov zoo. Qhov saum npoo ntawm qhov qis adsorption ntsis yog hydrophobic kho kom txo qis qhov nro ntawm cov kua tawm ntau qhov seem hauv lub taub. Cov lus qhia dav ntawm lub qhov ncauj yog qhov zoo tagnrho rau kev nqus cov khoom nplaum, genomic DNA, thiab cov kua dej ntawm tes.
Cov lus qhia txog kev ua tau zoo suav nrog kev tuav qis, lim caij, nruj, thauj khoom thiab tshem tawm quab yuam, DNase thiab RNase dawb, thiab pyrogen dawb. Yog li, koj puas paub dab tsi cov lus qhia zoo yuav tsum muaj tsawg kawg? Lub tswv yim zoo nyob ntawm qhov sib npaug, taper, thiab qhov tseem ceeb tshaj plaws, nqus. Wb tham txog taper ua ntej. Yog tias zoo, qhov haum nrog rab phom yuav zoo thiab qhov kev ntshaw yuav raug ntau dua; concentricity yog ib tug ncaj saib ntawm lub taub hau saum toj kawg nkaus thiab qhov twg lub taub txuas mus rau lub pipette. Txawm hais tias nws yog concentric los yog tsis, yog tias nws tsis yog concentric, nws txhais tau hais tias concentricity tsis zoo; cov adsorption muaj feem xyuam rau cov khoom ntawm lub taub, thiab cov khoom tsis zoo ntawm lub taub hau yuav cuam tshuam rau qhov tseeb ntawm pipetting, uas ua rau muaj ntau cov kua residue los yog dai rau ntawm phab ntsa kom luv luv, uas ua rau pipetting yuam kev; yog li ntawd, Thaum xaiv cov lus qhia, koj yuav tsum tau xyuam xim tshwj xeeb rau peb lub ntsiab lus saum toj no.
Yuav ua li cas kuv yuav xaiv ib tug lim tswv yim nrog lim tip? "Raws li ntev raws li cov lus qhia uas tuaj yeem ntsia tau yog cov lus qhia uas tuaj yeem siv tau", qhov no yog qhov kev nkag siab zoo ntawm yuav luag txhua tus neeg siv ntawm kev hloov pauv ntawm cov lus qhia. Cov lus no yog arguably ib nrab tseeb tab sis tsis yog tag nrho. Koj tuaj yeem xav ua qhov kev xeem ua haujlwm tom qab sib piv cov pipette mus rau lub ntsis. Tom qab yaug qhov ncauj, rov ua qhov sib ntxiv ob peb zaug, ntsuas txhua qhov sib ntxiv thiab sau cov ntawv nyeem. Raws li qhov ceev ntawm cov kua yuav tsum tau ntsuas thiab hloov mus rau hauv ntim, qhov tseeb thiab qhov tseeb ntawm kev ua haujlwm pipetting tuaj yeem suav tau. Peb tab tom yuav xaiv cov saum toj kawg nkaus nrog qhov raug zoo. Yog tias qhov tseeb ntawm cov kav dej thiab cov kav dej tsis zoo, nws txhais tau hais tias qhov nruj ntawm cov pipette thiab cov pipette tsis tuaj yeem lav tau, yog li cov txiaj ntsig ntawm txhua qhov kev ua haujlwm tsis tuaj yeem rov ua dua. Yog li ntawd, xaiv ib tug zoo sai xav tau cov ntaub ntawv los hais.
