Kev siv lub raj mis serum
Lub raj mis yog tsim los ntawm cov iav borosilicate uas tsis tshua muaj extraction, thiab lub raj mis tsis muaj xim tsis muaj xim raws li qhov xav tau ntawm USP Hom I thiab ASTM E 438 Hom I Standard Class A. Nws tuaj yeem ua autoclaved ntawm 121 degree rau 20min, thiab tuaj yeem muab faib ua 125ml. , 250ml, 500ml, 1L, 2L raws li ntim.
Lub raj mis yog lub thawv rau khaws cov ntshav, uas feem ntau yog ua los ntawm cov khoom siv PET los ntawm kev txhaj tshuaj stretch tshuab. Qhov nruab nrab yog cov khoom noj uas xav tau rau kev loj hlob ntawm tes. Raws li nws qhov chaw, nws tau muab faib ua hluavtaws nruab nrab thiab ntuj nruab nrab, ob qho tib si tuaj yeem khaws cia rau hauv cov fwj tshuaj.
Lub raj mis
Ntuj nruab nrab:
Qhov feem ntau siv tej yam ntuj tso nruab nrab yog serum, yeej calf serum. Serum muaj ntau yam kev loj hlob ntawm tes, adhesion-txhawb yam tseem ceeb thiab lawv cov khoom siv ntau yam. Ua ke nrog cov khoom nruab nrab, cov hlwb tuaj yeem proliferate thiab loj hlob zoo. Cov ntshav yuav tsum tau muab khaws cia rau hauv lub raj mis tshwj xeeb ntawm -5 degree mus rau -20 degree .
Synthetic nruab nrab:
Synthetic nruab nrab yog nruj me ntsis raws li hom thiab kom muaj nuj nqis ntawm cov khoom xav tau los ntawm cov hlwb. Muaj ntau ntau hom thiab paub cov khoom, uas ua rau kev tswj xyuas qhov kev sim. Nws muaj cov carbohydrates, amino acids, lipids, inorganic ntsev, vitamins, kab kawm thiab lwm yam kev loj hlob ntawm tes. Txawm li cas los xij, piv nrog lub ntuj nruab nrab, qee qhov tsis paub txog lub ntuj tsis tuaj yeem hloov los ntawm cov khoom siv tshuaj paub. Yog li ntawd, cov khoom siv hluav taws xob yooj yim siv hauv kab lis kev cai ntawm tes yuav tsum tau ntxiv qee qhov ntawm cov khoom nruab nrab ntawm ntuj los kov yeej cov kab lis kev cai hluavtaws. Lub hauv paus tsis txaus, feem ntau kev xyaum yog ntxiv calf serum.
Cov duab ntawm lub raj mis yog square, yooj yim rau tuav, resistant rau feem ntau acid thiab alkali corrosion, embrittlement kub yog -70 degree, thiab nws tseem muaj ib tug tej yam toughness ntawm -30 degree . Nws yog lub thawv ntim khoom zoo, txawm tias nws yog ntuj nruab nrab lossis hluavtaws kab lis kev cai. tuaj yeem khaws cia rau hauv cov fwj dej ntshiab.
Rau kev sib cais, kab lis kev cai thiab kev txheeb xyuas ntawm tib neeg txoj hlab ntshav hlab ntsha endothelial progenitor hlwb thiab tsim cov acellular vascular scaffolds los ntawm kev txhim kho khov-thaw.
Txhawm rau tshawb nrhiav qhov ua tau thiab kev kho mob tus nqi ntawm cov kab mob endothelial progenitor cais thiab kab lis kev cai los ntawm tib neeg cov hlab ntsha hauv cov ntshav.
Cov hau kev: Cov ntshav qaum ntawm cov fetuses uas tau txais tag nrho lub sij hawm caesarean seem hauv Department of Obstetrics thiab Gynecology, Thawj Tsev Kho Mob ntawm Bengbu Medical College tau sau, muab tso rau hauv lub raj mis uas muaj 50U / ml heparin Nyob rau hauv aseptic tej yam kev mob, khaws cia nyob rau hauv ib tug 4 degree ice box, thiab thauj mus rau lub hlwb Nyob rau hauv lub chav kab lis kev cai, lub umbilical qaum cov ntshav mononuclear hlwb tau txais los ntawm qhov ceev gradient centrifugation. Cov hlwb mononuclear tau muab faib ua ob ntu, thiab fetal calf serum M199 nruab nrab (FCS-M199) thiab autologous serum M199 nruab nrab (AS-M199) tau ntxiv. Thiab lawv tau cog rau hauv kab lis kev cai tais diav nrog thiab tsis muaj tib neeg fibronectin (HFN) (cim li: F(0), F(1), A(0), A(1)) feem. Hauv vitro induction, sib txawv thiab nthuav dav tau ua.
Cov kev hloov morphological ntawm cov hlwb adherent thaum lub sij hawm tag nrho induction thiab txawv txheej txheem tau pom, thiab lawv tau txheeb xyuas los ntawm ntau lub ces kaum ua ke nrog lwm yam kev kawm xws li immunohistochemistry, immunofluorescence thiab txaus cytometry.
Cov txiaj ntsig: Tom qab 12 hnub ntawm kab lis kev cai, qaum cov ntshav mononuclear hlwb pib ua raws. Tom qab 3d, cov duab spindle pib tshwm, thiab tom qab ntawd tus naj npawb ntawm cov hlwb adherent maj mam nce thiab maj mam ua spindle-puab. Thaum kab lis kev cai mus txog rau ib lub lim tiam, ib qho kev sib koom ua ke tsim nrog spindle hlwb nyob ib ncig ntawm lub hlwb nyob hauv nruab nrab. Thaum cov hlwb raug coj mus rau 14 hnub, cov kab ke sib cuam tshuam ntawm tes maj mam txuas thiab tsim cov qauv zoo li lub network. Nyob rau tib lub sijhawm, nrog kev nce zuj zus ntawm lub sijhawm kab lis kev cai, cov kab mob spindle ntev kuj pib maj mam luv, thiab pom qhov hloov pauv zoo li pob zeb paving.
Cov hlwb adherent tau coj mus kuaj rau ib lub lim tiam thiab nws tau pom tias cov xov tooj ntawm cov hlwb hauv nruab nrab nrog tib neeg fibronectin tau nce ntau dua li qhov tsis muaj tib neeg fibronectin, thiab tsis muaj kev hloov pauv loj ntawm cov hlwb hauv ob qhov sib txawv nruab nrab. #Instrument consumables#
