Tus kab mob khaws cov kua dej yog cov kua tiv thaiv ntxiv rau hauv lub raj tshuaj tua kab mob txhawm rau txhawm rau tiv thaiv lub qog nasopharyngeal swab. Nws feem ntau xa mus rau hauv tsev raws li cov kab mob khaws cia ua kua, feem ntau hu ua VTM kua lossis UTM kua.Usually, thaum lub sijhawm kuaj nucleic acid, nucleic acid PCR tsis tuaj yeem ua ncaj qha rau ntawm qhov chaw khaws cov qauv, thiab cov hleb coj mus kuaj yuav tsum tau thauj mus rau kev sim, thiab VTM xav tau ntxiv.
Kev kuaj pom tus kab mob yog txawv ntawm kev kuaj pom tseeb biochemical. Tus kab mob nws tus kheej yog cov kab mob yooj yim uas yuav tsum tau nyob hauv xov tooj ntawm tes nyob tom qab. Tom qab cov qauv sim, tus kab mob tawm ntawm lub cell host, thiab nws lub plhaub protein thiab nucleic acid yuav ua rau ploj mus sai hauv lub raj xa ntshav. Yog li, thaum lub caij sim nucleic acid, nws tsis tuaj yeem txiav txim siab seb thawj tus qauv muaj tus kab mob, uas ntxim yuav ua rau tsis zoo.
Tom qab ntxiv cov kab mob kev tiv thaiv kev daws teeb meem rau lub raj xa dej ua qauv, cov teeb meem saum toj no tuaj yeem daws tau:
Tus kab mob khaws cia rau kev daws teeb meem yog qhov tsis ua haujlwm thiab tsis siv tshuaj
1. Cov kab mob khaws cia cov tshuaj tiv thaiv hom. Qhov nruab nrab ntawm cov tshuaj sib xyaw no muaj cov kab mob tua tau lysis zoo heev, tuaj yeem tiv thaiv tus kab mob sai sai tom qab ua piv txwv, zoo tiv thaiv kev pheej hmoo ntawm kev kis mob thib ob, thiab tso cov kab mob viral nucleic acid rau kev nrhiav kom yooj yim.
2. Kev daws teeb meem VTM muaj Rnase inhibitor, uas tuaj yeem tiv thaiv qhov tsis zoo nyob hauv cytoplasm los ua kom tsis muaj kab mob viral nucleic acid tom qab lub hlwb tau tawg thiab ua rau lub cev tawg. Cov dej num loj dua ntawm kev tiv thaiv tuaj yeem tiv thaiv kev ruaj ntseg ntawm viral nucleic acid thiab zoo heev txhim kho kev ua haujlwm ntawm nucleic acid tshem tawm.
3. Cov khoom lag luam VTM tuaj yeem khaws cia rau hauv chav tsev kub txog 12 lub hlis yam tsis muaj kev sim, thiab cov qauv tuaj yeem khaws cia ntawm chav sov rau ib lub lim tiam tom qab coj mus kuaj, nrog kev sib khi zoo, kev yooj yim thiab kev thauj mus los, thiab txuag nqi kev thauj mus los.
Txawv los ntawm cov hom tsis tseem ceeb saum toj no, cov tshuaj tiv thaiv kab mob tsis muaj tshuaj tiv thaiv kab mob tsis muaj lysis tsis haum, tab sis muaj ntau hom kev sib xyaw uas ua rau cov kab lis kev cai ntawm tus kab mob hlwb, xws li Hanks tov puag, gentamicin, tshuaj tua kab mob fungal, BSA (V) , cryoprotectant, lom neeg tsis tuaj yeem thiab cov amino acids, thiab lwm yam .This VTM tuaj yeem lav qhov muaj zog ntawm tus kab mob thiab pab txhawm rau txhawm rau lub sijhawm muaj sia thiab kev tiv thaiv tus kab mob ruaj khov. Yog li, kev tiv thaiv cov kab mob tiv thaiv thiab ntsuas qhov kub thiab txias ntawm cov qauv tom qab cov qauv yog yuav tsum muaj ntau dua.Non-inactivated tus kab mob tso kua mis tuaj yeem siv rau kev sib sau thiab thauj ntau ntau yam, nrog rau ntau yam kab mob thiab mycoplasma, ureaplasma, chlamydia , thiab lwm yam.
Txawm hais tias cov tshuaj tiv thaiv kab mob tsis siv tshuaj los yog tsis ua haujlwm, nws txoj haujlwm yooj yim yog tiv thaiv kev ncaj ncees ntawm tus kabmob lossis tus kabmob nucleic acid, kom ntseeg tau qhov tseeb ntawm kis tus kab mob nucleic acid, ob VTM muaj qhov zoo.
